2 edition of Molecular characterization of a methanogenic toluene-degrading consortium. found in the catalog.
Molecular characterization of a methanogenic toluene-degrading consortium.
Cheryl E. Washer
Written in English
Molecular techniques were used to characterize an enriched methanogenic toluene-degrading consortium of microorganisms, to identify putative genes (referred to as bssABE) which code for enzymes involved in the first step of toluene mineralization by this community, and to gain information about relationships between putative genes and organisms identified. A cloning study revealed that two organisms, Eub-1 and Eub-6, had survived in the consortium over an 8 year period, suggesting an important role for each of these organisms in the community. Degenerate primers designed from known bss operon sequences were used to identify at least two putative bssA and one putative bssBE. To our knowledge, this is the first report of putative bssABE sequences obtained from a methanogenic community. The presence of two putative bssA sequences may suggest the presence of two different toluene-activating organisms, a hypothesis supported by results from a quantitative PCR study of the consortium.
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Dr. Sabine Kleinsteuber Senior Scientist Head of the MicAS Group Department of Environmental Microbiology Working Group Microbiology of Anaerobic Systems Helmholtz Centre for Environmental Research - UFZ Permoserstr. 15, Leipzig, Germany Phone +49 [email protected] Enrichment and characterization of a sulfate-reducing toluene-degrading microbial consortium by combining in situ microcosms and stable isotope probing techniques FEMS Microbiol. Ecol. 71 .
Phenotypic and genotypic characterization of phenanthrene degrading fluorescent Pseudomonas biovars. Applied Environ. Microbiol., PubMed | Juhasz, A.L. and R. Naidu, Bioremediation of high molecular weight polycyclic aromatic hydrocarbons: A review of the microbial degradation of benzo[a]pyrene. Int. Biodeterior. A bacterium, strain BC, was isolated from a benzene-degrading chlorate-reducing enrichment culture. Strain BC degrades benzene in conjunction with chlorate reduction. Cells of strain BC are short rods that are μm wide and 1 to 2 μm long, are motile, and stain gram negative. Strain BC grows on benzene and some other aromatic compounds with oxygen or in Cited by:
Isolation and Characterization of Alicycliphilus denitrificans Strain BC, Which Grows on Benzene with Chlorate as the Electron Acceptor Sander A. B. Weelink, 1 Nico C. G. Tan, 1, † Harm ten Broeke, 1 Corné van den Kieboom, 1 Wim van Doesburg, 1 Alette A. M. Langenhoff, 2 Jan Gerritse, 2 Howard Junca, 3 and Alfons J. M. Stams 1, *. Enhanced biofilm production by a toluene-degrading Rhodococcus observed after exposure to perfluoralkyl acids. Published in Environ Sci Technol., Download paper here. Weathers T.S., Higgins C.P., Sharp J.O.
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Ficker M, Krastel K, Orlicky S, Edwards E. Molecular characterization of a toluene-degrading methanogenic consortium. Appl Environ Microbiol Anaerobic degradation of toluene and o-xylene by a methanogenic consortium Article (PDF Available) in Applied and Environmental Microbiology 60(1).
The enriched consortium has been previously shown to degrade high concentrations (> μM) of benzene rapidly (within 2 weeks). Mineralization was confirmed by the release of 14 CO 2 from radiolabeled benzene.
Also, the role of sulfate as the terminal electron acceptor was demonstrated by the stoichiometric balance between benzene loss and sulfate by: Polycyclic aromatic hydrocarbons (PAH) such as naphthalene are widespread, recalcitrant pollutants in anoxic and methanogenic environments.
A mechanism catalyzing PAH activation under methanogenic conditions has yet to be discovered, and the microbial communities coordinating their metabolism are largely unknown.
This is primarily due to the difficulty of Cited by: 2. The benzene ring moiety is found in biological compounds accounting for ~25% of the land-based organic matter on Earth. Hence, the biosynthesis and biodegradation of aromatic ring compounds constitutes an important part of natural carbon cycle.
Microorganisms capable of using aromatic compounds as carbon and energy sources can be found in a range of very different habitats Author: Weimin Sun, Valdis Krumins, Donna E. Fennell, Lee J. Kerkhof, Max M.
Häggblom. Ficker M, Krastel K, Orlicky S, Edwards E () Molecular characterization of a toluene-degrading methanogenic consortium. Appl Environ Microbiol. Chan, Winnie (). Characterization of Reductive Dehalogenases in a Chlorinated Ethene-Degrading Bioaugmentation Culture.
Moore, Eve (). Developing a Protocol to for the Enrichment and Characterization of a Methanogenic Toluene-degrading and Benzene-degrading Community. Chan, Peter (). Abstract. Polycyclic aromatic hydrocarbons (PAH) are widespread in methane-rich subsurface environments, such as oil reservoirs and fuel-contaminated aquifers; however, little is known about the biodegradation of these compounds under methanogenic by: Phenotypic tests and molecular characterization, based on 16S rRNA gene sequencing, showed that KA and KB belong to the Pseudomonas genus.
Phylogenetic analysis indicated that they were closely related to the Pseudomonas putida group. The highest sequence similarity (KA % and KB 99% identity) was found with P.
putida KT, GB1 and PD1 strains. The Cited by: Ficker M, Krastel K, Orlicky S, Edwards E () Molecular characterization of a toluene-degrading methanogenic consortium. Appl Environ Microbiol – PubMed Google Scholar Fischer A, Herklotz I, Herrmann S, Thullner M, Weelink SAB, Stams AJM, Schlomann M, Richnow HH, Vogt C () Combined carbon and hydrogen isotope fractionation.
Isolation and characterization of toluene-degrading bacteria. Soil samples that were obtained from either the northern end (N soil) or the southern end (S soil) of a C5+-contaminated soil pile (11, 14) were combined into two large samples of ca.
g ts (1 g) were incubated with 10 ml of MS medium and an F tol of either 1, 10, or %. Appropriate dilutions of these cultures Cited by: The strong-and increasing-interest in microbial biofilms, and the special properties connected with this life form, is related, in part, to their importance in medical and industrial contexts and, in part, to the unexpected complexity of the processes that apparently lie behind their development; the three-dimensional structures of many biofilms indicate organized buildup programs, which Cited by: 7.
This study investigated the performance and stability of an anaerobic baffled filter reactor in the treatment of algae-laden water from Taihu Lake at several organic loading rates. The study also evaluated the capability of soft filler to train granule sludge and improve the anaerobic environment and sludge activity in the anaerobic baffled reactor (ABR), thereby enhancing the Cited by: Toluene-degrading bacteria were isolated from the trickle bed air biofilter (TBAB), which had eliminated toluene efficiently for three months.
Among the isolates, Acinetobacter genospecies Tol 5 demonstrated the highest efficiency in toluene removal. genospecies Tol 5 tended to adhere to hydrophobic materials showing by: More important, a benzene-degrading consortium which was dominated by Acidovorax spp.
was detected in groundwater sample W18i, and t-RFLP community profiles indicate that the community is very similar to that in situ suggesting that members of the Acidovorax can degrade benzene in the SIReN aquifer (Fahy et al.
).Cited by: Characterization of acid-tolerant H 2 /CO 2-utilizing methanogenic enrichment cultures from an acidic peat bog in New York State Molecular characterization of a sulfate-reducing consortium which mineralizes benzeneCited by: Summary Toluene is a model compound used to study the anaerobic biotransformation of aromatic hydrocarbons.
Reports indicate that toluene is transformed via fumarate addition to form benzylsuccinate or by unknown mechanisms to form hydroxylated intermediates under methanogenic conditions.
We investigated the mechanism(s) of. Furthermore, a toluene-degrading methanogenic consortium was characterized by rRNA techniques. The consortium comprised two archaeal kinds related to the genera Methanosaeta and Methanospirillum, and two bacterial kinds, one related to the genus Desulfotomaculum and the other unrelated to any previously described genus.
Identification of Desulfosporosinus as toluene-assimilating microorganisms from a methanogenic consortium. International Biodeterioration and Biodegradation, A. Cupples. RDX degrading microbial communities and the prediction of microorganisms responsible for.
Edwards EA, Grbic-Galic D. Anaerobic degradation of toluene and o-Xylene by a methanogenic consortium. Applied and Environmental Microbiology ;60(1) RC (Final).
Indole and its derivatives, including 3-methylindole and 4-chloroindole, are environmental pollutants that are present worldwide. Microbial degradation of indole and its derivatives can occur in several aerobic and anaerobic pathways; these pathways involve different known and characterized genes.
In this minireview, we summarize and explain the microbial degradation Cited by: AIST HokkaidoTsukisamu-Higashi, Toyohira Ward, Sapporo City, Hokkaido, Japan + AIST Tsukuba Central 6 biodegradation of toluene hydrocarbon from gasoline polluted soil.
After initial screening, 28 different strains were isolated from soil samples and the most promising strain was selected for toluene degradation study. Biochemical and morphological characterization classified the bacterial strains selected as Pseudomonas sp. and was designatedFile Size: KB.